English to Russian Medical Medical: Pharmaceuticals

Cryopreservation
After 3 to 4 days culture, medium in 260ml flasks and 650ml flasks were analyzed by HPLC (Gel Filtration chromatography) and 9 clones having antibody concentration greater than 10 mg/L were further propagated in multiple 650 flasks in the same way as above.
For preparation of cryovials, cell suspension was transferred to 50 ml centrifugation tube, centrifuged at 200 g for 5 min., supernatant was discarded and pellet was resuspended in 10 ml of medium.
Cell count was performed and cells were cryopreserved with 10 X106 cells/ml/vial in 5 or 10 vials in freezing medium (10%DMS0 + 90% CDCHO medium) and finally stored at -196oC in liquid nitrogen.
From the nine clones, one high priority clone was selected and fed batch shake flask studies were performed.
For the fed batch shake flask studies, high priority clone was seeded in shake flask with seeding density 2X105 cells/ml in 200ml medium (CDCHO + 25µM MSX) + 15% feed i.e. CHOCD Efficient Feed BTM (Gibco).