Jun 4, 2017 03:49
7 yrs ago
10 viewers *
English term
multiplex single-base primer extension
English to Spanish
Medical
Genetics
This is from a text on cancer genome. I've "minisecuenciación múltiple" as a possible equivalent, but I'm not quite sure.
"Both assays rely upon multiple PCR (polymerase chain reaction) steps to amplify DNA regions of interest and multiplex single-base primer extension with dideoxynucleotides. Extension products are then analyzed either by mass spectrometry , which can distinguish different bases according to their mass-to-charge (m/z) ratio (Sequenom Mass Spectrometry system) ..."
I'd really appreciate any help you can provide.
"Both assays rely upon multiple PCR (polymerase chain reaction) steps to amplify DNA regions of interest and multiplex single-base primer extension with dideoxynucleotides. Extension products are then analyzed either by mass spectrometry , which can distinguish different bases according to their mass-to-charge (m/z) ratio (Sequenom Mass Spectrometry system) ..."
I'd really appreciate any help you can provide.
Proposed translations
(Spanish)
| 5 | amplificación multiplex de cebador de base única |
David Balayla
|
| 3 | extensión/amplificación multiple de cebadores/iniciadores/primeres de una sola base |
Neil Ashby
|
Proposed translations
4 days
David Balayla
Spain
Spain
Native in English
&
Spanish
Works in field
Spain
Translation & Communication
Native in: English (Variant: British) 
, Spanish (Variant: Standard-Spain) , Catalan (Variants: Western, Oriental, Valencian, Central)
Works in: English to Spanish, Spanish to English, English to Catalan, and 5 more.
Related KudoZ points
:
12
Selected
amplificación multiplex de cebador de base única
La técnica se denomina 'minisecuenciación'.
Puedes utilizar 'de base única' o 'de una sola base'. Esa parte no es tan importante como el utilizar 'amplificación' para traducir 'extension'. Se usa también 'elongación' pero 'amplificación' es el término habitual. 'Multiplex' se utiliza habitualmente en el campo. 'Primer' es 'cebador' en biología molecular. En química/bioquímica quizá se utilice más 'iniciador', no lo sé.
Puedes utilizar 'de base única' o 'de una sola base'. Esa parte no es tan importante como el utilizar 'amplificación' para traducir 'extension'. Se usa también 'elongación' pero 'amplificación' es el término habitual. 'Multiplex' se utiliza habitualmente en el campo. 'Primer' es 'cebador' en biología molecular. En química/bioquímica quizá se utilice más 'iniciador', no lo sé.
4 KudoZ points awarded for this answer.
Comment: "Thanks, David!"
6 hrs
Neil Ashby
Spain
Spain
Native in English
Works in field
Spain
DrNeil TechTrans, Precision & Clarity
Native in: English
Works in: Spanish to English, English
Related KudoZ points
:
8
extensión/amplificación multiple de cebadores/iniciadores/primeres de una sola base
Amplificación Múltiple Dependiente de Sonda (Multiplex Ligation-dependent Probe Amplification o MLPA): permite amplificar varias secuencias objetivo con un único par de cebadores, evitando así las limitaciones de resolución de la PCR multiplex.
https://es.wikipedia.org/wiki/Reacción_en_cadena_de_la_polim...
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Note added at 8 days (2017-06-12 08:33:23 GMT) Post-grading
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Thanks Kevin! Very just and fair of you.
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Note added at 8 days (2017-06-12 08:34:43 GMT) Post-grading
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I give the same answer 4 days earlier, with a more common term ("multiple" as opposed to "multiplex"), a real reference that actually opens and that's the thanks I get.
https://es.wikipedia.org/wiki/Reacción_en_cadena_de_la_polim...
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Note added at 8 days (2017-06-12 08:33:23 GMT) Post-grading
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Thanks Kevin! Very just and fair of you.
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Note added at 8 days (2017-06-12 08:34:43 GMT) Post-grading
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I give the same answer 4 days earlier, with a more common term ("multiple" as opposed to "multiplex"), a real reference that actually opens and that's the thanks I get.
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