Sep 15, 2020 18:08
4 yrs ago
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English term
Sequences were collapsed
English to Russian
Science
Biology (-tech,-chem,micro-)
Extracted genomic DNA was amplified in a bias-controlled multiplex PCR, followed by high-throughput sequencing. Sequences were collapsed and filtered in order to identify and quantitate the absolute abundance of each unique TCRβ CDR3 region for further analysis.
Proposed translations
(Russian)
3 | количество последовательностей сокращали [путем слияния/объединения] | Igor Andreev |
Proposed translations
2 hrs
Selected
количество последовательностей сокращали [путем слияния/объединения]
Цитата из статьи, на которую ссылается статья https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418734/#R24, в которой присутствует подобный текст (Sequences were collapsed and filtered in order to identify and quantitate the absolute abundance of each unique TCRβ CDR3 region for further analysis as previously described (Robins 2009, Robins 2012, Carlson 2013).) с объяснением процедуры:
Finally, a nearest neighbor algorithm was used to collapse the data into unique sequences by merging closely related sequences, to remove both PCR error and GA sequencing error
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774550/
В выделенном фрагменте говорится о сокращении объема данных секвенирования за счет слияния гомологичных последовательностей и выделения на их основе уникальных
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Comment: "Спасибо"
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