English to Italian Science Chemistry; Chem Sci/Eng

Figure 3 shows the mean number of conditioned gapes elicited by a LiCl-paired saccharin
solution among the rats pretreated with various doses of CBDA (n = 8 per group) or HU-580 (n = 8
per group) or just with vehicle (VEH; n = 8).

For analysis of data from the acute nausea experiment (In vivo experiment 1), a single factor
ANOVA was conducted for the mean number of gapes in the 2 min test, and subsequent pairwise
comparisons were assessed with least significant difference (LSD) post hoc tests. For analysis of
data from the anticipatory nausea (AN) experiment (In vivo experiment 2), a single factor ANOVA
was conducted for the number of gapes in the 5 min AN test and for the total distance travelled in
the activity test, and subsequent pairwise comparisons were assessed with LSD post hoc tests.

The mean number of conditioned gaping responses was measured during
the anticipatory nausea test trial. Each bar represents the mean ± SEM. *P < 0.05, significant
difference from the VEH-treated control animals.

For in vivo experiment 2, contextually elicited conditioned gaping (a model of anticipatory
nausea) was measured using a distinctive conditioning chamber made of opaque black Plexiglass
(22.5 x 26 x 20 cm) with an opaque lid that sat on a table with a clear glass top. A mirror beneath
the chamber at a 45° angle facilitated viewing of the ventral surface of the rat to observe orofacial
responses. The conditioning chamber was in a dark room next to a 25 W light source.