Glossary entry (derived from question below)
English term or phrase:
rescue it into live virus
English answer:
use it to make live virus
Added to glossary by
Alami
Jun 7, 2010 16:49
14 yrs ago
8 viewers *
English term
rescue
English
Medical
Medical (general)
It is although possible to chemically synthetise the variola virus genome and rescue it into live virus without access to an inventoried variola sample.
What is meant by "rescue it" in this context? transform or insert?
Thank you in advance!
What is meant by "rescue it" in this context? transform or insert?
Thank you in advance!
Responses
Responses
2 hrs
Selected
use it to make live virus
rescue it into live virus – use it to make live virus
Smallpox virus is almost extinct. Stocks of it are held only by the Americans and the Russians. This would be a good project for terrorists or totalitarian governments looking for an alternative to making nuclear weapons. In theory, it is remarkably easy. First, they synthesise a small amount of the smallpox virus DNA chemically. Then, they infect cells with another poxvirus and shortly afterwards transfect the synthetic smallpox virus DNA into the infected cells. As the infection proceeds, the poxvirus replicates its own DNA and makes new virus particles as usual. This DNA is packaged into the particles to complete the replication process. However, at the same time, the synthetic smallpox virus DNA is also recognised by the genetic system of the other poxvirus. So the synthetic smallpox virus DNA is also replicated and smallpox virus particles are made, based on the synthetic smallpox virus DNA, but using the genetic mechanisms of the other poxvirus. The newly made smallpox virus DNA is packaged into the newly made smallpox virus particles, which results in live, that is infectious, smallpox virus.
Here is an introduction to the problem:
http://news.bbc.co.uk/2/hi/2122619.stm
Here is an article about it:
http://www.plospathogens.org/article/info:doi/10.1371/journa...
And here is how to do it:
http://jvi.asm.org/cgi/content/full/77/13/7281
4 KudoZ points awarded for this answer.
Comment: "thanks a lot to all of you, and especially to Mr. Nicholson!"
3 mins
insert/introduce/ inoculate
; )
22 mins
make it become active (or alive)
I am not sure if viruses are considered living beings today but I believe the text is saying that you can chemically synthesize a virus in a lab (the variola virus in this case) and get it to become active, or "alive." I personally think this is science fiction but I may be completely outdated. ;)
32 mins
3 hrs
the 'recovery' of live virus through... systhesis of viral genome
I hope this is the right context :-)
The first starting point is that certain kinds of biological engineering, including making pathogens drug resistant, and recovering live viruses from transfected recombinant DNA, are technically feasible and have been so for a very long time. The recovery of polio virus from cDNA was accomplished by Baltimore and coworkers in 1982.
The first starting point is that certain kinds of biological engineering, including making pathogens drug resistant, and recovering live viruses from transfected recombinant DNA, are technically feasible and have been so for a very long time. The recovery of polio virus from cDNA was accomplished by Baltimore and coworkers in 1982.
Example sentence:
chemically synthetise the variola virus genome and engineer live virus
Recovery of infectious classical swine fever virus (CSFV) from full-length genomic cDNA clones by a swine kidney cell line expressing bacteriophage T7 RNA polymerase
22 hrs
ensure replication / facilitate generation
I believe that virus rescue is slightly more than introduction or recovery; it is a process whereas virus replication/generation is facilitated. A few examples for the use of the term in context:
"... to ensure that the generated recombinant viruses were able to replicate. (...) system can be used for the generation of high yields of influenza B virus"
http://www.pnas.org/content/99/17/11411.full
"This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes."
http://www.ncbi.nlm.nih.gov/pubmed/10516084
"... we use a plasmid–only rescue system that allows the rapid generation of high–yield recombinant vaccine strains."
http://rstb.royalsocietypublishing.org/content/356/1416/1965...
"... to ensure that the generated recombinant viruses were able to replicate. (...) system can be used for the generation of high yields of influenza B virus"
http://www.pnas.org/content/99/17/11411.full
"This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes."
http://www.ncbi.nlm.nih.gov/pubmed/10516084
"... we use a plasmid–only rescue system that allows the rapid generation of high–yield recombinant vaccine strains."
http://rstb.royalsocietypublishing.org/content/356/1416/1965...
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