Glossary entry (derived from question below)
English term or phrase:
clearing of the background lawn
English answer:
disappearance (dying) of the homogeneous bacterial growth
Added to glossary by
Jörgen Slet
Nov 5, 2003 09:16
21 yrs ago
5 viewers *
English term
Estonia
19 years of biomedical translation
Native in: Estonian 
Works in: English to Estonian, Estonian to English
clearing of the background lawn
English
Science
molecular biology
At least five different amounts of test chemical are tested, with half-log intervals between plates. Substances are tested up to the limit of solubility or toxicity. Toxicity is evidenced by a reduction in the number of spontaneous revertants, a clearing of the background lawn, or by degree of survival of treated cultures. Non-toxic chemicals should be tested to 5 mg per plate before considering the test substance negative.
Responses
| 5 +1 | as for the previous question... |
achisholm
|
| 4 | merged colony |
Transflux (X)
|
Responses
+1
16 mins
Selected
as for the previous question...
Bacteria are plated out with the test agent (perhaps in top agar as before, or perhaps spread over the surface of the nutrient plates). In the control plates (no agent) the bacteria will grow and will form a "lawn" - a homogeneous growth over the plate (this appears as a cloudy, sometimes "fluffy" surface. Depending on the test agent, growth will be eggected and so growth will be less (less cloudy lawn) or more (more cloudy lawn). At a certain concentration, all growth will be killed of and so the lawn will be cleared.
In other tests, plates with an already grown bacterial lawn have disks soaked in agents placed on them and their effect on growth will result in clear patches developing on the plate die to the bacteria being killed off (i.e. the lawn being cleared).
In other tests, plates with an already grown bacterial lawn have disks soaked in agents placed on them and their effect on growth will result in clear patches developing on the plate die to the bacteria being killed off (i.e. the lawn being cleared).
4 KudoZ points awarded for this answer.
2 hrs
merged colony
according to "Dorlands Medical Dictionary"
lawn
an area sown with a thick growth of living substance
bacterial lawn
the confluent blanket of merged colonies resulting from using a concentrated bacterial solution
lawn
an area sown with a thick growth of living substance
bacterial lawn
the confluent blanket of merged colonies resulting from using a concentrated bacterial solution
Discussion