Jun 10, 2019 08:00
5 yrs ago
English term

DNA ligase for matching sequences

English to Italian Tech/Engineering Engineering (general)
Come tradurreste il passaggio "DNA ligase for matching sequences"?

"Other systems capable of short sequence reads include SOLiD™ and Ion Torrent technologies (both sold by Applied Biosystems™). SOLiD™ technology employs sequencing by ligation. Here, a pool of all possible oligonucleotides of a fixed length are labelled according to the sequenced position. Oligonucleotides are annealed and ligated; the preferential ligation by DNA ligase for matching sequences results in a signal informative of the nucleotide at that position. Before sequencing, the DNA is amplified by emulsion PCR. The resulting bead, each containing only copies of the same DNA molecule, are deposited on a glass slide. The result is sequences of quantities and lengths comparable to Illumina sequencing."



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DNA ligasi per sequenze corrispondenti

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