Glossary entry (derived from question below)
English term or phrase:
semipermeable surface columns
Russian translation:
колонки с полупроницаемой поверхностью фазы
Added to glossary by
Nina Nikitina
May 30, 2010 09:27
14 yrs ago
English term
semipermeable surface columns
English to Russian
Science
Chemistry; Chem Sci/Eng
ВЭЖХ
After hydrolysis, XXX resIdues are separated from the sample matrix by gel permeation chromatography, hexane/acetonitrile partitioning and high performance liquid chromatography using both reverse phase and *semipermeable surface columns*.
Как эти колонки по-русски называются?
Как эти колонки по-русски называются?
Proposed translations
(Russian)
4 +2 | колонки с полупроницаемой фазой |
Pavel Venediktov
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Proposed translations
+2
17 mins
Selected
колонки с полупроницаемой фазой
Наверное так и называть. Смысл в покрытии внутренней фазы внешним полупроницаемым слоем для исключения помехи от крупных молекул.
The Internal Surface Reversed Phase (ISRP)-an elegant invention of Dr. Thomas C. Pinkerton-was the first example of a Restricted Access Medium (RAM). These materials allow the determination of small molecules in the presence of large biomolecules, for instance the direct injection of serum into an HPLC column without requiring prior removal of protein. A new RAM is the Semipermeable Surface (SPS), based on the doctoral research of Carla Desilets under Dr. Fred Regnier at Purdue University, developed to its present form at the Regis Chemical Company. The SPS has two phases: an outer and an inner, each independently synthesized, each covalently bound to the silica surface of the packing particle. The outer is a hydrophilic polyoxyethylene polymer; the inner is any of several common hydrophobic reversed phases (at present, C8, C18, nitrile, phenyl). The outer phase forms a semipermeable surface that prevents large biopolymers from reaching the inner phase. Small molecules, however, can and do interact with either the outer or the inner phase. They are retained by a unique mechanism-a combination of hydrogen bonding at the outer polyoxyethylene surface and hydrophobic interaction at the inner reversed phase. Aside from its experimentally variable selectivity-a completely new property-SPS also shows excellent chromatographic efficiency and roughly twice the retentivity of comparable RAM materials. http://goo.gl/r7sv
The Internal Surface Reversed Phase (ISRP)-an elegant invention of Dr. Thomas C. Pinkerton-was the first example of a Restricted Access Medium (RAM). These materials allow the determination of small molecules in the presence of large biomolecules, for instance the direct injection of serum into an HPLC column without requiring prior removal of protein. A new RAM is the Semipermeable Surface (SPS), based on the doctoral research of Carla Desilets under Dr. Fred Regnier at Purdue University, developed to its present form at the Regis Chemical Company. The SPS has two phases: an outer and an inner, each independently synthesized, each covalently bound to the silica surface of the packing particle. The outer is a hydrophilic polyoxyethylene polymer; the inner is any of several common hydrophobic reversed phases (at present, C8, C18, nitrile, phenyl). The outer phase forms a semipermeable surface that prevents large biopolymers from reaching the inner phase. Small molecules, however, can and do interact with either the outer or the inner phase. They are retained by a unique mechanism-a combination of hydrogen bonding at the outer polyoxyethylene surface and hydrophobic interaction at the inner reversed phase. Aside from its experimentally variable selectivity-a completely new property-SPS also shows excellent chromatographic efficiency and roughly twice the retentivity of comparable RAM materials. http://goo.gl/r7sv
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Comment: "Спасибо!"
Discussion
ссылок, а на "полупроницаемая мембрана" (в том же контексте) - 1347.
Я не спорю с Вами, и вовсе не собирался Вас поправлять. Просто, пока я подбирал ссылки получше "мембрану" Вы дали ответ про "фазу". Я
посчитал мой ответ не принципиально отличающимся от Вашего, и дал
(для Нины) еще один возможный вариант.